Topic

No. of hours

Lecture

Practical

1.Cellular structure.

5

5

2.Introduction: Background and

advantages of cell and tissue culture, and

biology of cultured cells.

5

5

3. Experimental animal handling.

5

5

4. Design and layout for a dedicated cell

culture lab.

5

5

5. Aseptic technique, culture vessels and

laboratory safety.

4

4

6. Cell culture media and requirements.

4

4

7. Serum free media.

4

4

8. Primary Cell culture.

4

4

9. sub culture and Cell lines.

4

4

10. Cell separation and characterization.

4

4

11. Cloning and Selection.

4

4

12. Cell Differentiation.

4

4

13. Transformation, immortalization,

contamination and cryopreservation.

4

4

14. Cell Quantitation.

4

4